Vulvar Anatomy Understanding the External Genitalia (PDF) The Bartholin's gland is located at the entrance to the vagina, and it secretes mucus, providing vulval and vaginal lubrication. A Bartholin's cyst occurs when the gland becomes blocked, and this can Female External Genitalia Human Reproduction: A Clinical Approach The Vulva Hormonally Bartholin Duct Cyst and Gland Abscess: Office Management AFP What can affect it!! Let's understand , Vulvar Health matters , Bartholin glands also known as greater vestibular glands are present under your vestibule or labia minora at 4 & 8 O'clock of vaginal